mom igg blocking solution (Vector Laboratories)

Vector Laboratories is a verified supplier

Vector Laboratories manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Vector Laboratories mom igg blocking solution
Mom Igg Blocking Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 7399 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mom+solution/Rabbit+IgG+(Control+Antibody)/pm40197319-193-24-28
Average 96 stars, based on 7399 article reviews

mom igg blocking solution - by Bioz Stars, 2026-09

96/100 stars

Images

Incubation:

Article Title: Amyloid precursor protein induces reactive astrogliosis
Article Snippet: .. Mouse brain sections were blocked with MOM solution (#MKB-2213, Vector Labs,) and then incubated overnight with primary antibodies against INFγ (1:100, #MCA1301, Bio-Rad), GFAP (1:1000, #ab53554, Abcam) and APP (1:200, #ab2072, Abcam) or against Aβ (MOAB-2 1:1000, #ab126649, Abcam). .. The next day, the sections were incubated with donkey anti-mouse (AF555, 1:500, #A3157, Thermo Fisher), donkey anti-goat (AF488, 1:500, #A11055, Thermo Fisher) and donkey anti-rabbit (AF647, 1:500, #A31573, Thermo Fisher) or donkey anti-mouse (AF555, 1:500, #ab150106, Abcam) secondary antibodies followed by DAPI staining and Mowiol mounting.

Article Title: Deficiency of endothelial sirtuin1 in mice stimulates skeletal muscle insulin sensitivity by modifying the secretome
Article Snippet: .. Sections were blocked in a MOM solution (MKB 2213 vector lab) for 1 h followed by further blocking in a solution containing 5% normal horse serum in PBS for 1 h. After blocking, the sections were incubated with CD31 antibody (550274,1:200 in 5% horse serum in PBS; BD Bioscience, San Diego, CA, USA), Sirt1 antibody (sc-15404, 1:1000 in 5% horse serum in PBS, Santa Cruz), anti-myosin BA-F8 IgG2b (1:250 in 5% horse serum in PBS), anti-myosin BF-F3 IgM (1:250 in 5% horse serum in PBS), anti-myosin SC-71 MIgG1 (1:250 in 5% horse serum in PBS, Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA, USA), and rabbit anti-mouse Laminin antibody (L9393,1:500 in 5% horse serum in PBS, Sigma) at 4 °C for approx. .. 12 h. The sections were then rinsed in PBST (1% Tween 20 in PBS, pH 7.4) and incubated for 1 h at room temperature with the following secondary antibodies: donkey anti-rat IgG(H + L) Alex 647(A-21247, 1:200 in PBST, ThermoFisher Scientific), goat anti-rabbit Alex IgG(H + L)568 (A-11011, 1:200 in PBST, ThermoFisher Scientific), goat anti-rabbit Alex IgG(H + L)488 (ab150077,1:200 in PBST, Abcam), goat anti-rabbit Alex IgG(H + L)405 (A31556B,1:200 in PBST, ThermoFisher Scientific), anti-mouse 647 IgG2B (A21242,1:200 in PBST, ThermoFisher scientific), anti-mouse 555IgG1 (A21127, 1:500 in PBST, ThermoFisher scientific), and anti-mouse 488 IgM (A21042,1:200 in PBST, ThermoFisher Scientific).

Article Title: A selective chemical probe for exploring the role of CDK8 and CDK19 in human disease
Article Snippet: Subsequently, they were placed in 2N HCl for 30 min at 37°C, rinsed thoroughly in PBS and then treated for a further 20 min at 37°C with 100μl pre-warmed 0.05% trypsin in PBS (Invitrogen). .. The sections were then rinsed with PBS and blocked in 100μl blocking solution from the Mouse-On-Mouse immunostaining kit (Vector Labs) for 1 hr, followed by incubation with 1:1000 anti-BrdU mouse monoclonal antibody (clone BU-33, Sigma B8434) in MOM solution (from the Mouse-on-Mouse immunostaining kit from Vector Labs) overnight at 4°C. .. This was followed by treatment with the biotinylated secondary antibody and ABC solution from the Mouse-On-Mouse immunostaining kit (Vector Labs) according to the manufacturer’s instructions.

Article Title: Amyloid precursor protein induces reactive astrogliosis.
Article Snippet: Images were acquired and processed using the ImageJ software.114 .. Mouse brain sections were blocked with MOM solution (#MKB- 2213, Vector Labs) and then incubated overnight with primary antibodies against INFγ (1:100, #MCA1301, Bio- Rad), GFAP (1:1000, #ab53554, Abcam) and APP (1:200, #ab2072, Abcam) or against Aβ (MOAB- 2 1:1000, #ab126649, Abcam). .. The next day, the sections were incubated with donkey anti- mouse (AF555, 1:500, #A31570, Thermo Fisher), donkey anti- goat (AF488, 1:500, #A11055, Thermo Fisher) and donkey anti- rabbit (AF647, 1:500, #A31573, Thermo Fisher) or donkey anti- mouse (AF555, 1:500, #ab150106, Abcam) secondary antibodies followed by DAPI staining and Mowiol mounting.

Article Title: A selective chemical probe for exploring the role of CDK8 and CDK19 in human disease
Article Snippet: Subsequently, they were placed in 2 N HCl for 30 min at 37 °C, rinsed thoroughly in PBS and then treated for a further 20 min at 37 °C with 100 μl pre-warmed 0.05% trypsin in PBS (Invitrogen). .. The sections were then rinsed with PBS and blocked in 100 μl blocking solution from the Mouse-On-Mouse immunostaining kit (Vector Labs) for 1 h, followed by incubation with 1:1,000 anti-BrdU mouse monoclonal antibody (clone BU-33, Sigma B8434) in MOM solution (from the Mouse-on-Mouse immunostaining kit from Vector Labs) overnight at 4 °C. .. This was followed by treatment with the biotinylated secondary antibody and ABC solution from the Mouse-On-Mouse immunostaining kit (Vector Labs) according to the manufacturer’s instructions.

Blocking Assay:

Article Title: Deficiency of endothelial sirtuin1 in mice stimulates skeletal muscle insulin sensitivity by modifying the secretome.
Article Snippet: .. Sections were blocked in a MOM solution (MKB 2213 vector lab) for 1 h followed by further blocking in a solution containing 5%normal horse serum inPBS for 1 h. After blocking, the sections were incubatedwith CD31 antibody (550274,1:200 in 5% horse serum in PBS; BD Bioscience, SanDiego, CA, USA), Sirt1 antibody (sc-15404, 1:1000 in 5% horse serum in PBS, Santa Cruz), anti-myosin BA-F8 IgG2b (1:250 in 5% horse serum in PBS), antimyosin BF-F3 IgM (1:250 in 5% horse serum in PBS), anti-myosin SC-71 MIgG1 (1:250 in 5% horse serum in PBS, Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA, USA), and rabbit anti-mouse Laminin antibody (L9393,1:500 in 5% horse serum in PBS, Sigma) at 4 °C for approx. .. 12 h. The sections were then rinsed in PBST (1% Tween 20 in PBS, pH 7.4) and incubated for 1 h at room temperature with the following secondary antibodies: donkey anti-rat IgG(H + L) Alex 647(A-21247, 1:200 in PBST, ThermoFisher Scientific), goat antirabbit Alex IgG(H + L)568 (A-11011, 1:200 in PBST, ThermoFisher Scientific), goat anti-rabbit Alex IgG(H+ L)488 (ab150077,1:200 in PBST, Abcam), goat anti-rabbit Alex IgG(H+ L)405 (A31556B,1:200 in PBST, ThermoFisher Scientific), anti-mouse 647 IgG2B (A21242,1:200 in PBST, ThermoFisher scientific), anti-mouse 555IgG1 (A21127, 1:500 in PBST, ThermoFisher scientific), and anti-mouse 488 IgM (A21042,1:200 in PBST, ThermoFisher Scientific).

Article Title: A selective chemical probe for exploring the role of CDK8 and CDK19 in human disease
Article Snippet: Subsequently, they were placed in 2 N HCl for 30 min at 37 °C, rinsed thoroughly in PBS and then treated for a further 20 min at 37 °C with 100 μl pre-warmed 0.05% trypsin in PBS (Invitrogen). .. The sections were then rinsed with PBS and blocked in 100 μl blocking solution from the Mouse-On-Mouse immunostaining kit (Vector Labs) for 1 h, followed by incubation with 1:1,000 anti-BrdU mouse monoclonal antibody (clone BU-33, Sigma B8434) in MOM solution (from the Mouse-on-Mouse immunostaining kit from Vector Labs) overnight at 4 °C. .. This was followed by treatment with the biotinylated secondary antibody and ABC solution from the Mouse-On-Mouse immunostaining kit (Vector Labs) according to the manufacturer’s instructions.

Staining:

Article Title: Spontaneous Cardiac Calcinosis in BALB/cByJ Mice
Article Snippet: Slides were washed in PBS (3 washes for 5 min each) and blocked by using antimouse blocking solution (MOM Solution, Vector Laboratories, Burlingame, CA) with 0.1% Triton X100, according to the manufacturer's instructions. .. Primary antibodies (rabbit antiCD3, catalog no. ab5690, Abcam, Cambridge, MA; rabbit antimyeloperoxidase, catalog no. RB375A1, Thermo Fisher Scientific, Waltham, MA; rat antiCD4, catalog no. 100402, Biolegend, San Diego, CA; rat antiCD8, catalog no. 100702, Biolegend; rat antiCD11b, catalog no. 64347, Abcam) were diluted 1:50 (MOM Solution, Vector Laboratories) for staining and analysis of paraffin-embedded sections as described earlier; prepared sections were visualized under light microscopy (Eclipse E800, Nikon). ..

Light Microscopy:

Immunostaining:

Article Title: A selective chemical probe for exploring the role of CDK8 and CDK19 in human disease
Article Snippet: Subsequently, they were placed in 2 N HCl for 30 min at 37 °C, rinsed thoroughly in PBS and then treated for a further 20 min at 37 °C with 100 μl pre-warmed 0.05% trypsin in PBS (Invitrogen). .. The sections were then rinsed with PBS and blocked in 100 μl blocking solution from the Mouse-On-Mouse immunostaining kit (Vector Labs) for 1 h, followed by incubation with 1:1,000 anti-BrdU mouse monoclonal antibody (clone BU-33, Sigma B8434) in MOM solution (from the Mouse-on-Mouse immunostaining kit from Vector Labs) overnight at 4 °C. .. This was followed by treatment with the biotinylated secondary antibody and ABC solution from the Mouse-On-Mouse immunostaining kit (Vector Labs) according to the manufacturer’s instructions.

Review